Zero order, First order derivative and Dual wavelength Spectrophotometric methods have been developed for simultaneous estimation of two component drug mixture of Ondansetron Hydrochloride and Pantoprazole Sodium in pure and bulk form. All these methods utilize methanol as a solvent. In Zero order spectrophotometry, absorbance of each solution of Ondansetron and Pantoprazole was measured at 303.80 nm and 288.80 nm respectively. In First order derivative spectroscopy method, absorbance of Pantoprazole Sodium was measured at 256.20 nm (Zero Crossing Point of Ondansetron Hydrochloride) and absorbance of Ondansetron Hydrochloride was measured at 289 nm (Zero Crossing Point of Pantoprazole Sodium). The drugs obeyed the Beer’s law in the concentration range of 10-60 µg/ml (r2 = 0.9994) for Pantoprazole Sodium and 1-6 µg/ml (r2 = 0.9998) for Ondansetron Hydrochloride. In Dual wavelength method, the determination of Ondansetron was carried out at the absorbance difference between 282.40 nm and 295.80 nm and of Pantoprazole at the absorbance difference between 296.20 nm and 308.80 nm. The results of analysis have been validated statistically. These methods were found to be simple, rapid and accurate, hence can be used for routine simultaneous estimation of these drugs in formulations. The results were found to be within acceptance criteria according to ICH Q2 R1 guideline.
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